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Legionella sample result interpreter

Your lab report has arrived with a number in CFU per litre. Enter it here with the type of sample and your building, and see which action band it falls in and exactly what to do next.

For hot and cold water systems, HSG274 Part 2 sets three response bands: under 100 CFU/litre is consistent with a system under control, 100 to 1,000 means review the control scheme and resample, and over 1,000 means immediate remedial action. Cooling towers, healthcare premises and spa pools work to their own regimes, which this tool applies for you. The bands are explained in full in sampling and action levels and what CFU levels mean.

The result
CFU/litre
What was sampled
The building
Who uses the water

The action levels this tool uses

The bands below are the ones set out across our guidance on sampling and action levels. “CFU/litre” (colony-forming units per litre, also written cfu/L) is the number of live legionella cells in the sample that grew into countable colonies.

SystemUnder controlReview and resampleImmediate action
Hot and cold water (outlets, calorifier, tank) — HSG274 Part 2Under 100 CFU/litre100 to 1,000 CFU/litreOver 1,000 CFU/litre
Cooling tower or evaporative condenser — HSG274 Part 1Up to 100 CFU/litreOver 100 and up to 1,000 CFU/litreOver 1,000 CFU/litre
Spa pool — HSG282Not detectedAny detection
Healthcare premises — HTM 04-01Works to lower thresholds than HSG274 Part 2; take any detection to your water safety group, whatever the count.

Three things the bands do not tell you. First, a result describes one sample from one point on one day — legionella lives in biofilm and its distribution is patchy, so a low count at one outlet is not an all-clear for the system. Second, “not detected” and a count under 100 are different results: one found nothing at the method's detection limit, the other found a little. Third, the response to a count is always to fix the system and prove the fix with another sample, not to watch the number.

Important

This tool is general guidance, not advice on your specific premises and not a laboratory interpretation service. It never states that your system is safe or compliant. For any result in the review or immediate-action bands, and always in healthcare premises, care homes, cooling towers and spa pools, involve a competent person, usually one working for a Legionella Control Association registered company. This site does not provide medical advice; if you are worried about someone's health, contact a doctor.

Important This page is general guidance only. Legionella risk varies with the specific water system, its use, and the people exposed to it. You should consult a competent legionella risk assessor for advice on your premises. LegionellaCheck is an independent information service and is not affiliated with HSE, UKAS, the Legionella Control Association, or any water hygiene company. This site does not provide medical advice. If you suspect Legionnaires' disease, contact NHS 111 or your GP.